- Amazon Room – closed during the visit, but Louise sneaks in with Regular Sized Rudy in tow
- Hands-on Room – contains the 'Sands from Exotic Lands' exhibit
- Pioneer Exhibit
The museum's director is Madeline Greenberg. Other employees include Jeff, an unseen employee, a guard who works in the Amazon room despite hating plants and numerous other employees who are part of the UFMWD (United Federation of Museum Workers and Docents) who are striking outside the museum.